Aim: Bluetongue is a non-contagious, infectious viral disease of domestic 
and wild ruminants; which is believed to have originated in Africa. The 
epidemiology of Bluetongue virus infection is poorly defined in many parts of 
the world, including a wide range of Asia and the Middle East. This paper 
reports the results of a Bluetongue serological survey in sheep from some 
provinces of Iran during 2007-2008. 
              
              Materials and Methods: A total of 996 
sheep sera were collected from 8 provinces in Iran and tested for Bluetongue 
virus specific using c-ELISA. Results: The results showed that the Bluetongue 
virus seroprevalence of sheep over the entire study areas was 34.93%, with the 
highest and lowest prevalence seen in West-Azerbaijan (64.86%) and Qom (12.1%) 
areas respectively. 
              
              Conclusion: The results demonstrated a high 
prevalence of Bluetongue antibodies in Iranian sheep, giving serological 
evidence of extensive exposure to Bluetongue virus infection in some provinces 
of the country. Keywords: antibodies, bluetongue virus, c-ELISA, Iran, 
seroprevalence